What is Renilla luciferase?
Renilla luciferase is an enzyme that catalyzes the chemical reaction which is responsible for the green bioluminescence of Renilla reniformis, a soft coral also known as sea pansy and a member of the class Anthozoa [1].
What is the difference between Firefly and Renilla luciferase?
What is the Difference Between Firefly and Renilla Luciferase? Firefly luciferase assays uses luciferin in the presence of oxygen, ATP and magnesium to produce light (Green/Yellow, 550-70 nM), while Renilla luciferase assays (SCT153) requires only coelenterazine and oxygen to produce light (Blue, 480 nM).
What is dual luciferase reporter assay?
The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.
What is the function of the luciferase enzyme?
Luciferases are proteins with enzymatic activity that, in the presence of ATP, oxygen, and the appropriate substrate (typically luciferin), catalyze the oxidation of the substrate in a reaction that results in the emission of a photon.
How does Renilla luciferase work?
Renilla luciferase, a monomeric 36kDa protein, catalyzes coelenterate-luciferin (coelenterazine) oxidation to produce light (3; Figure 1). Post-translational modification is not required for its activity, and the enzyme may function as a genetic reporter immediately following translation.
Who named luciferase?
Raphaël Dubois
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively.
Where is luciferin found?
Dinoflagellate luciferin is a chlorophyll derivative (i. e. a tetrapyrrole) and is found in some dinoflagellates, which are often responsible for the phenomenon of nighttime glowing waves (historically this was called phosphorescence, but is a misleading term).
How do you run luciferase assay?
Dispense 100µl of the Luciferase Assay Reagent into luminometer tubes, one tube per sample. 2. Program the luminometer to perform a 2-second measurement delay followed by a 10-second measurement read for luciferase activity. The read time may be shortened if sufficient light is produced.