What medium is used to culture Mycobacterium tuberculosis?

Mycobacterium tuberculosis is a slow-growing bacterium that is the etiological agent of tuberculosis. Agar-based and egg-based media incorporating green malachite and Middlebrook broths or solid media are recommended as the “gold standard” for isolation, culture, and definite diagnosis of M. tuberculosis (6).

Is Lowenstein Jensen medium solid or liquid?

Löwenstein-Jensen medium, better known as LJ medium, is a selective egg-based medium specifically used for the culture and isolation of Mycobacterium species, including Mycobacterium tuberculosis, from clinical specimens.

What is Jensen medium?

Jensens Medium is formulated according to Jensen and is recommended for detection and cultivation of nitrogen fixing bacteria (2). Sucrose acts as the energy source. Sodium molybdate in the media increases the fixation of nitrogen (3). Sodium chloride maintains osmotic equilibrium of the media.

Why is Jensen medium Lowenstein?

Lowenstein Jensen (LJ) Media is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria.

How can you distinguish Mycobacterium tuberculosis and Mycobacterium bovis?

Introduction. Unlike Mycobacterium tuberculosis, which only causes human tuberculosis (TB), M. bovis is a zoonotic pathogen that infects humans and domestic animals. This infection causes bovine TB, thus posing a major economic and public health problem for the animal husbandry industry.

How do I identify Mycobacterium?

Mycobacterial species are usually identified by time-consuming culture methods. Recently, the development of rapid diagnostic tests that use molecular genetic methods, such as PCR amplification, has been reported.

Why LJ medium is used?

Uses of LJ Medium It is used for the diagnosis of Mycobacterial infections. It is used for testing antibiotic susceptibility of isolates. It is also used for differentiating different species of mycobacterium (by colony morphology, growth rate, biochemical characteristics and microscopy).

Is Mycobacterium Gram negative or positive?

Mycobacteria are Gram-positive, catalase positive, non-motile, non-spore forming rod-shaped bacteria (0.2–0.6 μm wide and 1.0–10 μm long).