What is Q5 polymerase?
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
What is processivity of Taq polymerase?
Polymerase Processivity “Processivity” is a term that refers to the number of nucleotides incorporated by a polymerase in a single binding event (before dissociation). Taq DNA polymerase adds approximately 50 nucleotides per binding event (8).
Does Q5 produce blunt ends?
Blunt-ended products are produced by Q5 High-Fidelity DNA Polymerase (and all Q5 product formulations).
Is Q5 a Taq?
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates.
What is in Q5 buffer?
The 5X Q5 Reaction Buffer contains 2 mM MgCl2 at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer.
Does Taq polymerase have 5 to 3 exonuclease activity?
Taq DNA polymerase has a domain at its amino terminus (residue 1 to 291) that has a 5′-3′ exonuclease activity, a 3′-5′ exonuclease domain in the middle (residue 292 to 423), and a domain at its C-terminus that catalyzes polymerase reactions.
What is 3 ‘- 5 exonuclease activity?
The 3′–>5′ exonuclease activity intrinsic to several DNA polymerases plays a primary role in genetic stability; it acts as a first line of defense in correcting DNA polymerase errors. A mismatched basepair at the primer terminus is the preferred substrate for the exonuclease activity over a correct basepair.
What is high-fidelity DNA polymerase?
High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion high-fidelity DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase–like proofreading enzyme.
What do you understand with high fidelity and thermostable DNA polymerase?
High-fidelity DNA polymerases have several safeguards to protect against both making and propagating mistakes while copying DNA. Such enzymes have a significant binding preference for the correct versus the incorrect nucleoside triphosphate during polymerization.
Does DMSO inhibit PCR?
“DMSO is a PCR enhancer that increases yield and specificity in PCR.” DMSO is a kind of PCR enhancer that commonly utilized during the PCR reaction to optimize the reaction. Several other PCR enhancers are MgCl2, KCl, albumin, (NH4)2 SO4 and salts.
Why do we use DMSO?
DMSO is used topically to decrease pain and speed the healing of wounds, burns, and muscle and skeletal injuries. DMSO is also used topically to treat painful conditions such as headache, inflammation, osteoarthritis, rheumatoid arthritis, and severe facial pain called tic douloureux.
What is Q5 DNA polymerase?
Product Information Q5 ® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
When is the polymerase activated in Q5 hot start?
The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. Q5 Hot Start does not require a separate high temperature activation step, shortening reaction times and increasing ease-of-use.
What is the denaturation temperature of Q5 polymerase?
During thermocycling, the denaturation step should be kept to a minimum. Typically, a 5–10 second denaturation at 98°C is recommended for most templates. Optimal annealing temperatures for Q5 High-Fidelity DNA Polymerase tend to be higher than for other PCR polymerases.
How do I get a sample of Q5 high-fidelity DNA polymerase?
Choose from a Selection of Standalone Enzymes, Master Mixes and Kits Request a free sample of Q5 High-Fidelity DNA Polymerase at Q5PCR.com One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc.