What is M13 phage vector?
M13 is a filamentous bacteriophage which infects E. coli host. The M13 genome has the following characteristics: Circular single-stranded DNA.
What is the M13 bacteriophage used for?
Recently, M13 bacteriophage has started to be widely used as a functional nanomaterial for various electrical, chemical, or optical applications, such as battery components, photovoltaic cells, sensors, and optics. In addition, the use of M13 bacteriophage has expanded into novel research, such as exciton transporting.
What is M13 in microbiology?
M13 plasmids are used for many recombinant DNA processes, and the virus has also been used for phage display, directed evolution, nanostructures and nanotechnology applications. Escherichia virus M13.
Why is bacteriophage M13 useful as sequencing vector?
Lengths of DNA are cloned into the bacteriophage M13 that provides a source of large quantities of single-stranded DNA. This single-stranded DNA can then be used as a template in a primer extension dideoxynucleotide sequence reaction.
What is the nature of M13 phage DNA?
M13 phage is a bacterial virus composed of a single-stranded DNA encapsulated by various major and minor coat proteins. It has a long-rod filament shape that is approximately 880 nm in length and 6.6 nm in width (Smith & Petrenko, 1997).
How does bacteriophage M13 replicate?
It is concluded that in M13 infection semicon- servative RF replication of a double strand to a double strand, in contrast to single-stranded DNA synthesis, depends specifically on the dnaB function. semiconservative replication of double-stranded RF DNA.
How are M13 vectors created?
M13 cloning vectors have been developed for the selection of DNA sequences capable of directing initiation of DNA synthesis on single-stranded templates. These vectors are derived from viable M13 mutants containing large deletions in the region of the complementary strand origin.
Why M13 phage is used in phage display?
Numerous bacteriophage species have been employed in phage display systems, including f1, fd, T4, M13 and T7, of which the latter two examples are considered to be efficient display vectors. The most commonly employed phage display system is M13 as it contains nonessential regions that allow exogenous gene insertions.
How was M13 formed?
In this work, a bifunctional M13 strain was created in which a streptavidin-binding domain was added to the pIII receptor-binding protein at one end of the phage virion and a hexahistidine metal-binding domain was added to the pIX filament cap protein at the other end of the virion.
Is M13 phage lytic or lysogenic?
lysogenic phages
Phages are divided into three main classes based on their production and generation: lytic phages such as T4; temperate phages like Lambda; and lysogenic phages such as M13. M13 is a filamentous phage that converts the host cell into a generation factory without lytic disruption.
What are the major coat proteins in M13 phage that are used in phage display?
The M13 DNA is coated by major coat protein pVIII which has 2,700 copies in the mature phage and has 50 amino acid residues. The pVIII is coded by a single gene with the same name (gVIII). The minor coat proteins include pIII and pVI at one end and pVII and pIX at the other end of the phage.
What does the M13 fragment in a phagemid contain?
What does the M13 fragment in a phagemid contain? Explanation: These signal sequences are recognized by the enzymes that convert the normal double-stranded M13 molecule into single-stranded DNA before secretion of new phage particles.