What is anti-GFP antibody?

What is anti-GFP antibody?

Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.

Which antibody is tagged with GFP?

GFP tag Monoclonal Antibody (1E10H7)

Can GFP detect antibodies?

Green fluorescent protein (GFP) is a 27 kDa monomeric protein, isolated from the jellyfish Aequorea Victoria that auto-catalytically forms a fluorescent pigment. GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.

What is anti-GFP Nanobody?

Abstract. The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and is emerging as a powerful tool for isolation and cellular engineering of fluorescent protein fusions in many different fields of biological research.

Does GFP antibody recognize BFP?

NSJBio’s anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

What is immunostaining used for?

Immunostaining is used in cell biology to study differential protein expression, localization and distribution at the tissue, cellular, and subcellular level.

Do GFP antibodies recognize EGFP?

Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.

Is GFP used in immunofluorescence?

Epitope tags and anti-epitope tag antibodies are widely used for purification and detection of recombinant proteins in immunoblotting, immunostaining and immunoprecipitation.

Is green fluorescent protein an antibody?

Abstract. We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg).

Can GFP antibody detect EGFP?

A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.

What is a Chromobody?

A Chromobody is a genetic fusion of a target-specific VHH from alpaca and a fluorescent protein (TagRFP or TagGFP, EGFP). The Chromobody is available in form of a plasmid or selected stable cell lines (not shown).

What is the function of anti GFP antibody?

GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields.

What is the dilution of anti-GFP tag mouse monoclonal antibody?

Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.

Does ab183734 bind to GFP?

Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab183734 was applied gave a stronger signal in the 488 channel, indicating that ab183734 is binding to GFP and therefore eliciting signal amplification.

What proteins does the XP rabbit Mab detect?

GFP (D5.1) XP ® Rabbit mAb detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein.