What are the differences between nephelometry and turbidimetry?
In nephelometry the intensity of the scattered light is measured, while, in turbidimetry, the intensity of light transmitted through the sample is measured. Nephelometric and turbidimetric measurements are used in the determination of suspended material in natural waters and in processing streams.
What is the principle of nephelometry?
The principle of nephelometry and turbidimetry is based on the scattering or absorption of light by solid or colloidal particles suspended in solution. When light is passed through the suspension, part of incident radiant energy is dissipated by absorption, reflection, and reaction while remainder is transmitted.
What are the components of nephelometry?
The basic components of a nephelometer include (1) a light source, (2) collimating optics, (3) a sample cell, and (4) collection optics, which include light-scattering optics, detector optical filter, and a detector.
What is the difference between a spectrophotometer and a nephelometer?
Nephelometry is concerned with measurement of scattered light from a cuvette containing suspended particles in a solution. The components of a nephelometer are the same as a light spectrophotometer except that the detector is placed at a specific angle from the incident light.
What is nephelometry test used for?
Quantitative nephelometry is a lab test to quickly and accurately measure levels of certain proteins called immunoglobulins in the blood. Immunoglobulins are antibodies that help fight infection. This test specifically measures the immunoglobulins IgM, IgG, and IgA.
Is nephelometry an immunoassay?
Nephelometric immunoassay for the determination of drug levels in blood is based on the inhibition of immunoprecipitation by a hapten (drug). It represents a homogeneous method that does not require any separation steps nor radioisotopes.
Which detector is used in nephelometer?
A nephelometer or aerosol photometer is an instrument for measuring the concentration of suspended particulates in a liquid or gas colloid. A nephelometer measures suspended particulates by employing a light beam (source beam) and a light detector set to one side (often 90°) of the source beam.
How is nephelometry test done?
It is based on the principle that a dilute suspension of small particles will scatter light (usually a laser) passed through it rather than simply absorbing it. The amount of scatter is determined by collecting the light at an angle (usually at 30 and 90 degrees).
What is CU in turbidity?
The unit CU is defined as the negative decadic logarithm of the change in light intensity. This means: an increase in measured value of 1 CU corresponds to a 90% degradation of the light beam.
What is the principle of turbidity?
Turbidity is an optical property of water based on the amount of light scattered and absorped by collodial and suspended particles. The turbidity value measured in FNU, FTU, NTU etc. is the quantitative statement of this qualitative phenomenon.