How does lipofectamine increase transfection efficiency?
- The day before transfection, Plate 4×105 cells per well in 0.5 mL of complete growth medium.
- For each well of cells to be transfected, dilute 1 μg of DNA in 50 μL of Opti-MEM , and 2.5 μL of P3000 in 50 μL of Opti-MEM in separated tube.
- Mix gently and incubate 20 minutes at room temperature.
Is lipofectamine a transfection reagent?
Lipofectamine™ Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine™ Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.
Which transfection reagent is best?
Under the tested conditions, ViaFect™ Reagent offered the best combination of transfection efficiency and low toxicity for most cell lines, making it an ideal choice when beginning transfection experiments with a new cell line.
What is Lipofectamine p3000 reagent?
Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7)
Is lipofectamine 2000 toxic to cells?
I have found that in high concentrations, lipofectamine is toxic to HeLa cells, and as previously mentioned, the cell number seeded has an impact on this.
Does lipofectamine expire?
According to ThermoFisher, lipofectamine 3000 can be used until 2 years after the manufacturing date if stored properly (stored in a 4 degree fridge should be fine). The Optimem is sensitive to expiration. I would recommend using the new reagents for your experiment. Hope this helps!
Is Lipofectamine toxic to cells?
2.3. Although Lipofectin showed the lowest toxicity to HepG2 cells (89.54% viability), it displayed the lowest transfection efficacy too (8.29%). Generally, HepG2 cells displayed resistance to the toxicity of majority of the reagents tested, with Lipofectamine 3000 (70.59 % viability), being the most toxic reagent.
Does Lipofectamine expire?
How long is Lipofectamine good for?
According to ThermoFisher, lipofectamine 3000 can be used until 2 years after the manufacturing date if stored properly (stored in a 4 degree fridge should be fine). The Optimem is sensitive to expiration. I would recommend using the new reagents for your experiment.