How do you count cells in a hemocytometer formula?
Methods. Use the following formula in order to calculate the number of cells you have in your suspension: (total cells counted)/(4 squares counted)*10-4*initial volume*dilution factor = total number of cells; Note: 10-4 is the volume of squares on the hemocytometer (0.1 mm3).
How is WBC count calculated?
Total leucocyte count Calculations:
- One large area is 1 x 1 mm, and the depth is 0.1 mm.
- Total area counted in 4 large squares = 4 x 1 x o.
- Y x 10/4 is the total WBC in the cell in 1 µL.
- Now dilution is 1:20.
- Number of WBC in 1µL = Y x 10 x 20/4 = Y x 50 = Total WBC count.
- Total TLC = counted cells (Y) x 50 = TLC/cmm.
How do you calculate dilution factor in WBC?
So, 0.5 part of blood is in 100 parts of fluid or, 1 part of blood is mixed in 200 parts of fluid Thus, dilution factor for RBC counting is 200. 0.5 part of blood is mixed in 10 parts of fluid So, 1 part of blood is in 20 parts of fluid Thus, dilution factor for WBC counting is 20.
Why do you multiply by 10000 when counting cells?
You can think of each large square as having a volume of 100nL. So you have to multiply by 10,000 in order to convert the number of cells in 100nL to the number of cells per mL.
How much volume is a hemocytometer?
Most hemacytometers count cells in 0.1 µl fixed volume where one cell exists at 104 cells/ml sample. The range of hemacytometer is approximately 105 cells/ml.
How do you calculate the dilution factor of a hemocytometer?
Dilution Factor = Total Volume (Volume of sample + Volume of diluting liquid) / Volume of sample. Total viable cells/Sample = Viable Cells/ml x The original volume of fluid from which the cell sample was removed. Volume of media needed = (Number of cells needed/Total number of viable cells) x 1000.
What is the rule used when counting cells in a haemocytometer?
The rule is to count all the cells in the middle and those on two lines. You choose which two lines to count (bottom, upper, left or right) just try to count all the time the same lines to reduce final counting errors and deviations. The point of this “rule” is to avoid double counting.