What is May Grünwald Giemsa stain used for?
May Grunwald-Giemsa (MGG) Stain is used for staining of blood, bone marrow smears and clinical cytological specimens. May Grunwald-Giemsa (MGG) staining method is used for morphological inspection and differential counting of blood cells.
What is the principle of Grunwald Giemsa stain?
May grunwald giemsa stain is Romanowsky stain. They are composed of two components azure B and Eosin Y. Azure B bind with anionic parts of cell-like DNA and stain them blue. While Eosin Y binds with cationic parts of cell-like protein, it stains them the red color.
What does Giemsa and May Grünwald stain in the blood smear?
May-Grunwald-Giemsa staining method is used for morphological inspection and differential counting of blood cells. May-Grünwald staining combines the effect of acidic eosin and alkaline methylene blue. Giemsa staining makes effect of azure. This staining stains all cellular components.
How do you make may Grunwald Giemsa stain?
Method
- Dissolve 0.3 g of May Grunwald dye in 100 mL absolute methanol in a 250 mL conical flask.
- Warm the mixture to 50°C in a water bath for a few hours and allow it to cool to room temperature.
- Stir the mixture on a magnetic stirrer and leave it stirring for 24 hours.
- Filter the mixture and stain is ready for use.
Which stain is used for Pap smear?
Papanicolaou stain
Papanicolaou stain (also Papanicolaou’s stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.
Why is Giemsa staining commonly used in malaria surveys?
Applications Giemsa stain Being a differential stain, Giemsa stain can be used to study the adherence of pathogenic bacteria to human cells, differentiating human cells as purple and bacterial cells as pink. It can be used for histopathological diagnosis of malaria and some spirochete and protozoan blood parasites.
Which stain is used for malaria parasite?
The Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. The classical staining procedure requires between 30 and 45 min.
Why is Giemsa stain used for malaria?
Being a differential stain, Giemsa stain can be used to study the adherence of pathogenic bacteria to human cells, differentiating human cells as purple and bacterial cells as pink. It can be used for histopathological diagnosis of malaria and some spirochete and protozoan blood parasites.
What are the components of the May Grünwald stain?
May-Grünwald is alcohol based and contains May-Grünwald’s eosin methylene blue and methanol (>85%). Giemsa is alcohol based and contains Giemsa’s azure eosin methylene blue, methanol (>50%) and glycerol.
What does Giemsa stain bind to?
DNA
Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining. It is a mixture of cationic thiazine dyes, most importantly azure B, and anionic eosin dyes such as eosin Y (figure 4.1) [16].
Which fixative is used for MGG stain?
May Grunwald’s Blood Stains are basic and acidic dyes which induce multiple colours when applied to cells. Methanol acts as a fixative and also as a solvent. The fixative does not allow any further change in the cells and makes them adhere to the glass slide.
Why Pap stain is used?
Pap stain is a universal stain used for gynecologic and non-gynecologic cytology smear. It is mainly used for oral and cervical cancer screening in asymptomatic population and in the follow up of patients with cancer. Pap test has decreased incidence of cervical cancer by 70% in developed countries.