What does bovine serum albumin do?
Bovine Serum Albumin (BSA) is used in a variety of laboratory applications including its function as a protein concentration standard, its function as a cell nutrient and its ability to stabilize enzymes during restriction digest.
Is Bovine Serum Albumin safe?
GHS Storage and Disposal Phrases: Multi-region format Page 2 03/02/2015 Revision: Page: 2 of 5 Bovine Serum Albumin Assay Reagent SAFETY DATA SHEET 03/06/2014 Supersedes Revision: 2.3 Harmful if swallowed. Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation or …
What foods contain bovine serum albumin?
BSA is a protein found in Beef and in milk.
How do you get bovine serum albumin?
Manufacturing Methods. Bovine serum albumin (BSA) is purified from serum using one of three different purification methods: cold-organic solvent fractionation, heat shock and ion exchange chromatography.
Is bovine serum albumin similar to human albumin?
The protein human serum albumin (HSA) is able to readily crystallize in the presence of trivalent cations, whereas this is not the case for the homologous protein in cattle, bovine serum albumin (BSA), although both have analogous functions as well as similar physicochemical properties.
What kind of protein is BSA?
Bovine serum albumin (BSA) is a globular protein (~66 kDa MW) that is used in numerous biochemical applications due to its stability and lack of interference with biological reactions. The BSA structure is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates.
Is BSA a hazardous?
Harmful if swallowed. Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation or skin absorption. May cause eye, skin, or respiratory system irritation.
Is egg albumin the same as human albumin?
Ovalbumin (45 kd), the principal protein of egg albumen, is a different protein from human serum albumin (67 kd). A distinct lactalbumin occurs in milk, and other albumins are found in muscles and plants. A person allergic to all albumins would be hard pressed to find sustenance.
Is bovine serum albumin in milk?
Bovine serum albumin is the third most abundant whey protein in milk after α-lactalbumin and β-lactoglobulin, amounting to up to 10% of total whey proteins.
Why is bovine serum albumin used for blocking?
Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter well.
What is human albumin made of?
Albumin is made from human plasma (part of the blood) which may contain viruses and other infectious agents. Donated plasma is tested and treated to reduce the risk of it containing infectious agents, but there is still a small possibility it could transmit disease.
What is the original source of bovine serum albumin?
Bovine serum albumin (BSA or “Fraction V”) is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments.