What are the steps for FNAC procedure?

During Procedure: You will be asked to change into a gown. The doctor will clean the skin where the needle is to be inserted. In some cases you will also be given a local anaesthetic to numb the area. A very thin, hollow needle with a syringe is gently inserted through the skin into the lump or organ.

How do you make FNAC slides?

Slide Preparation for Adequacy:

  1. Following the FNA, gently remove the needle from the syringe while holding the needle above the slide in case of accidental spillage of the specimen.
  2. Draw air into the syringe and replace the needle onto the syringe.
  3. Squirt only 1 – 2 drops to the center of one of the labeled slides.

What is FNAC technique?

Background: Fine Needle Aspiration Cytology (FNAC) is a simple, quick and inexpensive method that is used to sample superficial masses like those found in the neck and is usually performed in the outpatient clinic. It causes minimal trauma to the patient and carries virtually no risk of complications.

How is FNAC test performed?

The FNAC test is a simple, rapid, and low-cost test used to assess a specific condition or body region. It involves aspirating cells from a particular region of the body using a fine gauge needle. The sample is then sent to the lab, where it is examined under a microscope through several processes.

How many methods of FNAC are there?

For the spreading, there are two methods that are widely used and recommended for different types of material. The “one-step method” is preferred for scant and dense material, like the one taken from the majority of breast solid masses. The “two-step method” is used mostly for liquid and hemorrhagic material.

Which needle is used in FNAC?

Fine needle aspiration cytology (FNAC) entails using a narrow gauge (25-22G) needle to collect a sample of a lesion for microscopic examination. It allows a minimally invasive, rapid diagnosis of tissue but does not preserve its histological architecture.

Which staining is used for FNAC?

Routinely various stains like Haematoxylin and Eosin (H&E), Romanowsky and Pap have been used for staining the FNAC smears. Romanowsky stains are routinely used for staining the blood films and air dried cytological smears [2].

Which needle is used in Fnac?

What is the purpose of FNAC?

Fine needle aspiration cytology (FNAC) is used to take cells from palpable nodules in organs or tissues. The purpose of this examination is to categorise the type of lesion responsible for its occurrence.

Which stain used in FNAC?

What is the size of FNAC needle?

25-27-gauge needles
Concerning the needle size for thyroid fine needle aspiration cytology (FNAC), 25-27-gauge needles are generally used in Western countries. However, in Japan, the use of larger needles (21-22-gauge needles) is common.

What is NPV of FNAC?

Thus, fine-needle aspiration cytology (FNAC) is often performed instead. However, we have recently shown that while its positive predictive value (PPV) is high (89%), negative predictive value (NPV) of FNAC is rather low (37%) in irradiated necks [1. R.

What is the Fnac procedure?

The FNAC procedure is short and precise. A Radiologist may be present during the procedure to reinforce the team of professionals attending to you. Mammograms and CT scan imaging tests are sometimes used to discover abnormalities of organs deeper in the body.

What are the requirements for the success of Fnac?

THE PRACTICE OF FNAC  Success of FNAC depends on four fundamental requirement: 1. Samples must be representative of the lesion investigated. 2. Samples must be adequate in terms of cells & other tissue components 3.

What are the methods of fixation and staining in Fnac?

FIXATION & STAINING  2 fundamentally different methods of fixation & staining are used in FNAC: 1. Air drying followed by staining with a haematological stain such as MAY GRUNWALD- GIEMSA STAIN , Jenner-Giesma, Diff-Quik 2.

When was Fnac first used?

FNAC • It was first described in 1930 in USA & become popular in late 1950s • FNAC is most often for diagnostic of palpable mass lesions • Palpable lesions commonly sampled are : breast masses , enlarged thyroid , &superficial soft tissue masses.