How is allelic richness calculated?

Allele richness (also referred to as allelic diversity) is calculated as the average number of alleles per locus [1].

What is Amova used for?

AMOVA stands for Analysis of MOlecular VAriance and is a method to detect population differentiation utilizing molecular markers (Excoffier, Smouse & Quattro, 1992). This procedure was initially implemented for DNA haplotypes, but applies to any marker system.

How do I run Arlequin software?


  1. Download Arlequin35. zip to any temporary directory.
  2. Extract all files contained in Arlequin35. zip in the directory of your choice.
  3. Start Arlequin by double-clicking on the file WinArl35.exe, which is the main executable file.

What is private allelic richness?

The estimated private allelic richness is the number of private alleles expected in the population when random subsamples of size g are taken from each of J populations under consideration (Kalinowski, 2004).

How is allelic diversity measured?

In brief, here are just a few of the measures of genetic diversity:

  1. Based on the number of variants among the alleles.
  2. Polymorphism or rate of polymorphism (Pj)
  3. Proportion of polymorphic loci.
  4. Number of alleles (A) and allelic richness (As)
  5. Average number of alleles per locus.
  6. Based on the frequency of variant alleles.

What does AMOVA measure?

An AMOVA (Excoffier et al. 1992; Dupanloup et al. 2002) is similarly based on the calculation of sums of squares between and within groups of individuals. Therefore, these sums of squares can be used to find the clustering of populations into groups that has the lowest amount of genetic variation within groups.

What is Arlequin used for?

Arlequin provides methods to analyse patterns of genetic diversity within and between population samples.

How do I make an Arlequin input?

Now Go File, save/export data as, choose arlequin data format. It will ask you for a haplotype data file name to save, choose the name of your datafile and hit save, it will then ask you for an arlequin project file name, choose the same name (the file extension will be different), hit save.

What does polymorphic loci mean?

Definition of Term polymorphic loci (English) A genetic loci with two or more alleles, at which the most common form has a frequency not exceeding 0.95 in a given population. (

What is outlier loci?

Luikart et al. state, “Outlier loci are genomic locations (or markers or base pairs) that show behavior or patterns of variation that are extremely divergent from the rest of the genome (locus-specific effects), as revealed by simulations or statistical tests” [1].

What is allelic richness?

Allelic richness measures are also commonly presented in population genetic summaries (as the number of alleles in a given locus or the mean number of alleles per locus).

Does rarefaction have a negative or positive impact on allelic richness?

In our examples, the rarefaction technique yielded a number of negative contributions of individual populations to total allelic richness, and also positive contributions of populations devoid of any original allele (such as AB in Table 1 or DKSO in Table 2 ).

Does allelic richness affect gene diversity and differentiation?

In both species, allelic richness and gene diversity behave quasi-independently over the populations compared and a higher differentiation is observed in allelic richness compared to gene diversity.

Is there a paradox of negative contributions to allelic richness estimation?

The “paradox” of negative contributions is however avoided under rarefaction by the private allelic richness estimation of Kalinowsky (2004), which should then be preferred to the CTR of Petit et al. (1998).