How do you compare transcriptome?

To compare transcriptomes between any two species, a first step is to establish homologous relationships between proteins in the two species. A second step is to identify expression data obtained from experiments that are performed under similar conditions or tissue types.

What does the transcriptome tell you?

What can a transcriptome tell us? An RNA sequence mirrors the sequence of the DNA from which it was transcribed. Consequently, by analyzing the entire collection of RNA sequences in a cell (the transcriptome) researchers can determine when and where each gene is turned on or off in the cells and tissues of an organism.

What is the difference between transcriptome and genome?

In brief, the “genome” is the collection of all DNA present in the nucleus and the mitochondria of a somatic cell. The initial product of genome expression is the “transcriptome”, a collection of RNA molecules derived from those genes.

Is the transcriptome the same in all cells?

Transcriptomes Are Indicative of Gene Activity In multicellular organisms, nearly every cell contains the same genome and thus the same genes. However, not every gene is transcriptionally active in every cell — in other words, different cells show different patterns of gene expression.

What is transcriptomic study?

Transcriptomics is the study of the transcriptome—the complete set of RNA transcripts that are produced by the genome, under specific circumstances or in a specific cell—using high-throughput methods, such as microarray analysis.

What is the difference between RNA-seq and microarray?

The main difference between RNA-Seq and microarrays is that the former allows for full sequencing of the whole transcriptome while the latter only profiles predefined transcripts/genes through hybridization.

What is the difference between exome and transcriptome?

Exome refers to the part of the genome consisting of exons, which code information for protein synthesis while transcriptome refers to the sum total of all the messenger RNA molecules expressed in the genes of an organism.

Why do we do transcriptome analysis?

The transcriptome is the complete set of transcripts in a specific type of cell or tissue. Generally, the goal of transcriptome analysis is to identify genes differentially expressed among different conditions, leading to a new understanding of the genes or pathways associated with the conditions.

What is the difference between genome transcriptome and proteome?

The genome, transcriptome and proteome. The transcriptome is constructed by the process called transcription, in which individual genes are copied into RNA molecules. Construction of the proteome involves translation of these RNA molecules into protein.

Why is microarray better than RNA-Seq?