Can Salmonella reduce tetrathionate?
Salmonella reduces tetrathionate to thiosulfate, which it can further reduce to H2S, by using enzymes encoded by the genes phs and asr.
Why is Tetrathionate broth used for salmonella?
Tetrathionate Broth is recommended for the selective enrichment method of isolating Salmonella typhi and other salmonellae from faeces, sewage, etc. Organisms which reduce tetrathionate, such as salmonellae, flourish in the medium whilst many faecal organisms are inhibited1.
What is tetrathionate reductase?
The Salmonella enterica enterica serovar Typhimurium tetrathionate reductase is a membrane-bound enzyme containing a bis(guanylyl molybdopterin) cofactor [Casse72, HinojosaLeon86]. The enzyme has not been purified as of 2006.
What are some possible effects of infection by Salmonella typhimurium?
Possible signs and symptoms of salmonella infection include:
- Stomach (abdominal) cramps.
- Blood in the stool.
What is tetrathionate ion?
The tetrathionate anion, S. 4O 2− 6, is a sulfur oxoanion derived from the compound tetrathionic acid, H2S4O6. Two of the sulfur atoms present in the ion are in oxidation state 0 and two are in oxidation state +5. Alternatively, the compound can be viewed as the adduct resulting from the binding of S 2−
How do you make Tetrathionate broth?
Dissolve 46 g of the medium in one liter of purified water. Heat with frequent agitation and boil for one minutes to completely dissolve the medium. Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base.
Is Salmonella susceptible to ampicillin?
Among 83 Salmonella isolates, 98.8 % of the Salmonella spp. were susceptible to chloramphenicol and co-trimoxazole and about 97.6 % of the isolates were susceptible to ampicillin.
How is tetrathionate produced?
5B); specifically, tetrathionate is produced when inflammatory oxygen radicals react with thiosulphate derived from the hydrogen sulphide produced by fermentative gut commensals (Winter & Baumler, 2011).
What is the chain of infection for Salmonella?
When Salmonella bacteria are ingested, they pass through a person’s stomach and colonize the small and large intestine. There, the bacteria invade the intestinal mucosa and proliferate. The bacteria can invade the lymphoid tissues of the gastrointestinal tract and spread to the bloodstream.
What is the pathogenesis of Salmonella?
Pathogenesis. Pathogenic salmonellae ingested in food survive passage through the gastric acid barrier and invade the mucosa of the small and large intestine and produce toxins. Invasion of epithelial cells stimulates the release of proinflammatory cytokines which induce an inflammatory reaction.